赤芝孢子粉破壁前后多糖释放能力比较研究

Studies on Difference between Sporoderm broken and Nonbroken Spores of Ganoderma lucidum (Leyss. ex Fr.) Karst. by Polysaccharide Analysis

目的 :探讨一种较准确测定中草药中水溶性多糖的分析方法。方法 :改良苯酚 硫酸法测定多糖释放能力。结果 :37℃、沸水浴及水煮条件下 ,破壁比未破壁孢子的多糖释放能力分别强 118.4% ,87.5 %和 6 9.6 % ;多糖含量的测定RSD <1.3 %。结论

Objective:To compare the release ability of water soluble polysaccharides in sporoderm broken and nonbroken spores of Ganoderma lucidum , and establish a comparatively correct method for the determination and analysis of polysaccharide contents in Chinese herbs.Method:The release ability of water soluble polysaccharides was determined on the basis of phenol sulfuric acid modification in different conditions.Result:The release ability of polysaccharides of sporoderm broken spores was much grerter than that of nonbroken spores; and the phenol sulfuric acid modified cation method proved excellent in accuracy and reproducibility, with a relative error less than 1.5%.Conclusion:The spores should be wall wracked if used as a nutriment, or for extraction and analysis of their effective components. The method can be successfully used for the determination of polysaccharide contents in Chinese herbs or nutriments. [