植物激素对百合鳞片愈伤组织生长的影响

Influence of Hormone on Callus Growth of Nove Cento's Bulb

在植物细胞全能性学说的基础上 ,采用单因子实验 ,分析比较了细胞分裂素 6- BA,生长素 2 ,4 - D,生长素 NAA等 3种植物激素对路易圣特百合鳞茎愈伤组织生长的影响。实验结果表明 ,诱导愈伤的最佳培养基为 MS+6- BA( 0 .0 5mg.L-1) +2 ,4 - D( 1.0 mg.L-1) +NAA( 0 .1mg.L-1) ,6- BA的质量浓度为 1.0 mg.L-1时有利于芽的形成 ,NAA质量浓度为 0 .0 5mg.

On the basis of plant cell's totipotency theory, choose one factor trial and analyze the influence of three hormone, which are cell mitogen (6 BA) auxin (2,4 D) and auxin (NAA) to the callus growth of Nove Cento's bulb. Meanwhile compare the 6 BA with the other two kinds of hormone, and get the best agar medium to callus induction: MS+6 BA(0 05 mg\5L -1 )+2,4 D(1 0 mg\5L -1 )+NAA(0 1 mg\5L -1 ) When the concentration of 6 BA comes to 1 0 mg\5L -1 , it's good for the gernination of sprout and when the concentration of NAA is 0 05 mg\5L -1 , it's good to form new little bulb.