液相层析法分离制备地黄活性成分梓醇

Separation and Preparation of Active Component of Radix Rehmanniae Catalpol by HPLC

用液相层析法分离制备了地黄活性糖成分梓醇，低压柱层析：流量为20～22ml／min，洗脱剂为石油醚，乙酸乙酯-乙醇-水（8：2：1），高压柱层析：吸附剂ODSC18（10—40μm）以甲醇／水（10％）为洗脱剂，流速20～25ml／min，Φ40×300min，第10～12流份得到纯品梓醇，经测定含量达97％。本法方便、易行。

The active component of Radix Rehmanniae catalpol has been separated and prepared byHPLC,using petroleum ether or acetic ether-alcohol-water(8: 2:1) as an eluant in 20-22 ml/min of flow rate on a low pressure column,or using ODS C18 (10～40μm) as an absorbent,methol-water(10% )as an eluant in 20-50ml/min of flow rate(Φ40 × 300mm)on a high pressure column. The purified catalpol exists in the tenth-twelfth parts. It is found that its content is up to 97% through identification. This method is simple and available.