真鲷精液和精巢超低温冷冻保存

Cryopreservation of Red Sea Bream Pagrosomus major Spermatozoa and Testes

以精子的活率和游动时间为指标，研究了福建南部沿海秋冬季真鲷生殖群体精液和精巢超低温冷冻保存的方法和鲜精在不同盐度或酸碱度条件下的生理特性．结果表明，以０．８％的ＮａＣｌ和０．０５％的ＫＣｌ配制的稀释液适合于真鲷精液的冷冻保存．Ｋ＋有助于提高精子的活率．添加甘氨酸可延长精子的游动时间．甘油、二甲基亚砜（ＤＭＳＯ）和甲醇均可作为真鲷精液的抗冻保护剂，但以甘油和ＤＭＳＯ混合使用时的抗冻效果较好．冷冻精液的效果优于冷冻精巢．鲜精在弱碱环境和盐度为３０时平均活率最高，游动时间最长．

Spermatozoa and testes cryoprotective technique of red sea breach Pagrosomus major,which spawns from October to December in the coastal waters of South Fujian, was carried out and the physiological characteristics of fresh spermatozoa at various salinities and pH was also investigated. Both survial rate and swimming time of frozen-thawed spermatozoa were taken as criterions for the evaluation of the effects of cryopreservation. The results indicated that 0. 8% sodium chloride and 0. 5% potassium chloride were appropriate extenders. K+ was necessary for increasing the survival rate and glycine could prolong swimming time of frozen-thawed spermato ica. Althought glycerol, DMSO and methanol were appropriate cryoprotectants, the mixture of glycerol and DMSO was the most effective for the red sea bream spermatozoa. Freezing semen obtained better results than freezing testes. Fresh spermatozoa gave out the highest average survival rate and the longest swimming time at PH 8. 08 and salinity of 30.