摘要
本文以猪脾细胞浆为原料,采用制备电泳方法纯化了RO抗原。纯化的样品在PAGE上呈现单条区带,通过凝胶过渡测定分子量约为80KD,在SDS-PAGE上主要表现为60KD多肽带,Western blotting证明此60KD即为RO抗原的活性多肽。
This report described the purification of Ro antigen from pig spleen cytoplasm by preparative clectrophoresis. The purified Ro antigen showed only one strong band on PAGE and a major 60KD on SDS-PAGE, while its molecular weight was 80KD accrd-ing to gel filtration. Western blotting proved that the 60KD polypeptide was Ro antigenic polypeptide
出处
《河北大学学报（自然科学版）》
CAS
1991年第4期60-63,共4页
Journal of Hebei University(Natural Science Edition)
基金
国家自然科学基金资助课题
关键词
Ro抗原
制备电泳
猪脾细胞浆
Ro antigen,preparative electrophoresis, cytoplasm of pig spleen.
