摘要
本文以去污剂(SDS)-酚法自Eltor弧菌小川型、稻叶型菌株提取RNA。该制剂理化特性为:(?),紫外分光测定其吸收峰在257nm处,DNA含量小于核酸量的8％,苔黑酚反应阳性。该制剂能保护C_(57)BL小鼠抗Eltor弧菌及金黄色葡萄球菌的攻击。观察到这种保护作用与RNA组分有关,而且无种属特异性。动物实验表明,以Eltor弧菌RNA制剂处理之小鼠对小鼠S_100有显著抑制作用(P<0.001)。经该制剂处理的C_(57)BL小鼠巨噬细胞对Elto孤菌的吞噬作用较对照组显著提高(P<0.01)。结果提示这种Eltor弧菌RNA制剂有可能作为一种非特异性免疫刺激剂而加以应用。
In this article, we reported that the ribonucleic acid preparations could be extracted from V.Eltor serotype Ogawa and Inaba by using the method of phenol-sodium dodecyl sulfate. The physicaland chemical properties of the RNA preparations were: A260/A280>2 and A260/A230>1.5.The absorptionpeak in U. V. spectrophtometry was at 257 nm. The DNA content in the nucleic acid was lessthan 8 per cent. The Orcinol reaction was positive. The RNA preparation could protect the C_(87)BLmice from the Challenge of V. Eltor and Staphylococcus aureus. The protective effect was found tobe relative to RNA fraction and without species specificity. The animal experiment showed that tran-splantation of sarcoma_(180) in mice was significantly inhibited by the RNA preparations in the trea-ted group (P<0.001). Phagocytosis of macrophage of C_(57) BL mice treated by the RNA preparationson V. Eltor was significantly higher than that of the controls (P<0.01). These results suggestedthat the RNA preparations could be used as an nonspecific immunologic stimulant.
