以纤维连接蛋白为标记物对膀胱癌侵袭及转移机制的研究

FIBRONECTIN AS A BIOLOGICAL TUMOR MARKER FOR BLADDER CANCER

以纤维连接蛋白(Fn)为标记物,应用免疫组化技术对32例膀胱癌及10例正常膀胱组织中上皮基底膜、血管基底膜及平滑肌束膜进行检测.结果显示,正常膀胱组织中,上皮及血管基底膜、平滑肌束膜连续性完整.浸润型膀胱癌,上皮基底膜和平滑肌束膜中断、缺失普遍存在,相应部位癌细胞侵袭,Fn成份表达率下降;浅表型膀胱癌改变不明显,两者间有显著性差异.在2例T_4期膀胱癌中发现癌细胞侵袭小血管,相应部位基底膜及Fn成份缺失.提示,基底膜及平滑肌束膜受损程度,其Fn成份表达程度可作为评估膀胱癌侵袭,转移及预后判定的客观指标.

An immunohistochemical technique was used to study the invasion and metastasis in 32 specimens of bladder cancer with 10 normal cases served as controls. The loss of the epithelial basement membrane and the smooth muscle bunch membrane, the lower expression of their Fn were observed in 14 samples of invading bladder cancer. Some bloood vesseles were invaded by cancer cells in 2 cases of T4 invading cancer,the loss of blood vesselar basement membrane and the lower expression of it Fn were observed in this position. It is suggested that the basement membrane and the expression of it Fn might be considered as markers for invasion,metastasis and prognosis in bladder cancer.