凋亡相关基因bcl-xl在乳腺癌中的表达

Expression of apoptosis-related gene bcl-xl in breast carcinoma

为了探讨凋亡相关基因bcl-xl在乳腺癌中的表达及其临床意义，本文采用免疫络化SABC法和原位末端标记（TUNEL）法，分别对68例浸润性乳腺癌原发灶中bcl-xl蛋白及细胞调亡指数进行了检测。结果表现：bcl-xl蛋白在不同病理类型、年龄及临床分期中的阳性表达率无统计学差异（P＞0．05）；但在腋转移淋巴结＜4个组和≥4个组的阳性表达率分别为45％和71．4％（P＜005）；bcl-xl蛋白明性组和阳性组的平均凋亡指数分别为0．32％和0．18％（P＞0．05）。提示：bcl-xl蛋白与腋淋巴结转移有关，有可能作为乳腺癌预后判断的一个指标。

Objective: To study the expression of bcl-xl and its relation to clinical factors in breast carcinoma,bcl-xl protein and apoptotic index (AI) were detected. Methods: bcl-xl protein and AI were detectd respectively in 68 cases of breast carcinoma by using immunohistochemical method and TdT-mediat x-dUTP nick end labeling (Tunel) method. Results:the expression of bcl-xl was not significantly associated with the histopathologic type, age, PTNM stage and AI (P > 0.05). While bcl-xl expression in the group with rt 4 or ≥4 axillary metastatic lymph nodes was 45% and 71. 4 % respectively (P <0-05). Conclusion:The expression of bcl-xl is associated with the number of axillary positive lymplh nodes, indicating a tend to development and metastasis in breast carcinoma. bcl-xl protein may be a predictive factor for breast carcinoma.