摘要
通过分离纯化、培养特性和生化反应的观察、毒素溶血活性和最小致死量的测定及中和试验对正常和猝死黑山羊肠内容物中的魏氏梭菌进行了初步研究。结果表明 ,猝死黑山羊肠内容物魏氏梭菌的分离率为 10 0 % ,而正常黑山羊肠内容物的分离率只有 4 0 % ( 16/ 4 0 ) ;肠内容物中魏氏梭菌主要是A型 ;魏氏梭菌不同分离株在培养特性、生化反应。
Clostridium welchii isolated from intestine contents of normal and sudden death black goats is preliminarily studied by cultural performance and biochemical reaction,determination of hemolytic activity, minimum lethal dose and neutralization test of toxins.The results indicate that the isolating rates of Cl.welchii are 100% in intestine contents of sudden death black goats and 40% in normal black goats.Cl.welchii isolated from intestine contents of black goats is mainly type A and different isolates of Cl.welchii vary in cultural performance,biochemical reaction,hemolytic activity and minimum lethal dose.
出处
《山地农业生物学报》
2001年第2期99-105,共7页
Journal of Mountain Agriculture and Biology
关键词
黑山羊
肠内容物
魏氏梭菌
溶血活性
最小致死量
中和试验
猝死症
black goats
intestine contents
Clostridium welchii
hemolytic activity
minimum lethal dose
neutralization test
