水稻吸氮能力与氮素利用率的QTLs及其基因效应分析

QTLs underlying rice root to uptake NH_4-N and NO_3-N and rice N use efficiency at seedling stage

利用F2 (Palawan(IR4 2 )群体的RFLP标记连锁图 ,对水稻根系NH4 N和NO3 N吸收能力及植株氮素生理利用率进行QTL区间作图分析 ,在第 2和 5染色体上分别测得控制水稻根系NH4 N吸收能力的QTL各 1个。前者表现为显著的加性及部分显性效应 ,后者有显著的加性和显性效应 ;在第 5和 6染色体上测得控制水稻幼苗根系NO3 N的吸收能力的QTL各 1个 ,两者的基因效应主要表现为显性效应 ;在第 12染色体上检出控制稻苗氮素生理利用率的QTL 1个 ,具有显著的加性和显性效应。此外 ,通过表型值在各标记的两纯合基因型间的差异显著性检验 ,发现第4染色体上的标记RG91对水稻幼苗根系NH4

A molecular marker linkage map of F 2 population consisting of 104 RFLP markers was employed to identify the quantitative trait loci (QTLs), which control the abilities of rice root to uptake NH 4 N and NO 3 N and the N use efficiency at seedling stage in a hydroponic experiment Two QTLs linked with ability to uptake NH 4 N were detected One of them located on chromosome 2 showed additive and partial dominant gene effects and another on chromosome 5 showed additive and dominant gene effects Two QTLs associated with ability to uptake NO 3 N were identified One of them located on chromosome 5 and another on chromosome 6 both showed dominant gene effects One QTL located on chromosome 12 was found to underlying N use efficiency, which showed significant additive and dominant gene effects Furthermore, significant difference was found between means of two parental classes at the marker RG91 on chromosome 4 in the ability of root to uptake NH 4 N by t test