反相液相色谱中溶质保留参数间的定量关系研究

Study on Quantitative Relationship between Retention Parameters of Solutes in Reversed-Phase Liquid Chromatography

比较了Snyder经验公式中的K0w 与S和溶质计量置换保留模型 (SDM R)中溶质保留参数Z(1mol溶剂化溶质被溶剂化固定相吸附时 ,在其接触表面处释放出溶剂或置换剂的总摩尔数 )与lgI(与 1mol溶质对固定相的亲和势有关的常数 )之间的定量关系。SDM R中的两个参数间不仅对同系物溶质可以有很好的线性关系 ,而且对非同系物溶质也有很好的线性关系。其线性关系的好坏主要取决于这两个参数数值范围的大小 ,与文献中报告的线性关系取决于是否单因素影响溶质的保留和完全由统计学规律决定的结论不同。与Snyder经验公式中相对应的参数进行了比较 ,SDM R保留参数的线性范围比Snyder公式中参数的线性范围宽。

A quantitative relationship between lg I (a constant relating to the affinity of one mole of solute to stationary phase) and Z (total moles of organic solvent released at the contact region as one mole solute adsorbed by stationary phase) in the stoichiometric displacement model for retention (SDM R) of solute was compared with the corresponding relationship in Snyder's Equation in reversed phase liquid chromatography (RPLC) It was found that the relationship from SDM R was more exact than that from Snyder's Equation The linearity depends on the ranges of both lg? I and lg? K 0 w The broader the range, the better the linearity is This conclusion conflicts to that reported in references in which the linearity only exists either when one factor dominates solute retention, or when it is completely dominated by statistics The linear relationship in SDM R and in Snyder's Equation was found valid not only for homologues but also for non homologues