新型亲和膜色谱用于去除胆红素的研究

Novel Affinity Membrane Used for Bilirubin Removal

以纤维素膜为亲和基质 ,接枝后分别以聚赖氨酸和季铵盐为配基 ,制备了两种亲和膜介质 ,用于去除磷酸缓冲液 (pH 7 4)及人血白蛋白 (HSA)溶液中的胆红素。实验结果表明 ,该方法对磷酸缓冲液中胆红素的去除率能达到 70 %以上 ;对HSA溶液中的胆红素 ,其去除率稍低 ,但在较低浓度的HSA溶液中 ,也能获得高于 5 0 %的去除率。并对亲和膜与胆红素间的亲和作用进行了研究 ,考察了温度、流速、HSA浓度及胆红素初始浓度等相关条件对去除效果的影响。结果表明 ,在较高的温度条件下 ,去除效果较佳 ;在较高的初始浓度情况下 ,胆红素的吸附速率较大。

Bilirubin is a toxic substance In order to effectively remove it from the hepatic patients'blood, two novel affinity membranes were prepared These were prepared by chemically grafting on cellulose and immobilized with different ligands One kind of ligand was poly D lysine,the other one was quaternary ammonium salt Both affinity membranes were used for removal bilirubin from phosphate buffer and HSA solutions,and the effects of temperature,HSA concentration,adsorption time in static state experiment and flow rate in dynamic state experiment have been investigated The results indicated that the membranes could remove over 70% bilirubin from phosphate buffer and at least 50% from low concentration HSA solutions The results also indicated that the removal efficiency was better at higher temperature In the static state experiment,four hours can be selected as adsorption time In the dynamic state experiment,the flow rate can be properly increased.