摘要
利用高效液相色谱法分析测定青霉素裂解液中的主要成分 6 氨基青霉烷酸 (6 APA)、青霉素G钾和苯乙酸 ,并对副产物青霉噻唑酸进行了液相色谱的定性检测。结果表明 ,实验方法简便、快速 ,结果准确 ,可以用于 6 APA生产的质量控制。
A method for the determination of main composition in degraded products of penicillin, 6 aminopenicillanic acid (6 APA),penicillin G kalium(PGK), phenylacetic acid(PAA), and the by product benzylpenicilloic acid(BPA) by HPLC was studied. Separation conditions were as follows: Spherisorb C\-\{18\} column, 250 mm×4 6 mm i d , 10 μm; mobile phase: V (methanol)∶ V (0 004 mol/L KH\-2PO\-4 buffer, pH 4 5)=50∶50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of quality control.
出处
《色谱》
CAS
CSCD
北大核心
2001年第1期88-90,共3页
Chinese Journal of Chromatography
基金
国家"九.五"重点攻关课题! (96 0C0 2 0 2 0 1)
