丁酸钠对重组CHO细胞生长及产物EPO表达的影响

Effect of Butyrate on Cell Growth and Expression of EPO in Recombinant CHO Cells

在 CHO- S- SFM II无血清培养基中维持培养重组 CHO细胞 ,考查添加丁酸钠对细胞生长、糖代谢及产物 EPO表达的影响。丁酸钠浓度达到 2 mmol/L时完全抑制了细胞的生长 ,同时 ,丁酸钠的添加也降低了葡萄糖比消耗速率 ( 30 %左右 )。在本培养系统中 ,丁酸钠并不能促进产物的表达 ,但有助于维持 EPO表达的稳定性。

Recombinant CHO cells were cultured in CHO\|S\|SFM II.The effects of sodium butyrate on cell growth,glucose metabolism and EPO expression were investigated.Cell growth decreased with the addition of sodium butyrate in the serum\|free medium and was entirely inhibited at 2 mmol/L sodium butyrate. The specific rate of glucose utilization was also reduced by 30% in the presence of sodium butyrate.In addition,EPO expression was not facilitated by the addition of sodium butyrate,but its addition may be helpful to maintain the stability of EPO productivity.