摘要
Aim To use and modify the molecular histology technique that was first introduced by Hansmann et al and to investigate the cell origin and the clonality of Hodgkin/Reed Sternberg (H/R S) cell. Method Single H/R S cells were isolated by micromanipulation from frozen histological sections of tissues affected by Hodgkins disease. After DNA was extracted from these cells, PCR was performed with primers designed for β globin gene that exists in any somatic cells. Results The results showed that a special PCR product (268bp) was found in 12 out of 38 H/R S cells (positive rate of 31.6% ) with DNA from the cells micropicked from the frozen sections, whereas no any products was found with the DNA from these cells of formalin fixed and paraffin embedded sections. Conclusions The molecular histology technique we used is suitable for detecting the immunoglobulin heavy and light chain gene rearrangement in single H/R S cells.
Aim To use and modify the molecular histology technique that was first introduced by Hansmann et al and to investigate the cell origin and the clonality of Hodgkin/Reed Sternberg (H/R S) cell. Method Single H/R S cells were isolated by micromanipulation from frozen histological sections of tissues affected by Hodgkins disease. After DNA was extracted from these cells, PCR was performed with primers designed for β globin gene that exists in any somatic cells. Results The results showed that a special PCR product (268bp) was found in 12 out of 38 H/R S cells (positive rate of 31.6% ) with DNA from the cells micropicked from the frozen sections, whereas no any products was found with the DNA from these cells of formalin fixed and paraffin embedded sections. Conclusions The molecular histology technique we used is suitable for detecting the immunoglobulin heavy and light chain gene rearrangement in single H/R S cells.
出处
《遵义医学院学报》
2001年第1期5-6,共2页
Journal of Zunyi Medical University
关键词
单细胞
组织
DNA提取
聚合酶链反应
polymerase chain reaction single cell histological section
