寡核苷酸探针检测龈下菌斑中伴放线菌放线杆菌的分布

Distribution of Actinobacillus actinomycetemcomitans in subgingival plaques in the patients with chronic periodontitis

目的 :研究伴放线菌放线杆菌在牙周炎患者和健康人龈下菌斑中的分布。方法 :利用合成的寡核苷酸探针对 6 0例慢性牙周炎患者 6 0患病位点、10例健康人的 10个对照位点龈下菌斑中伴放线菌放线杆菌进行检测。结果 :6 0个患病位点中有 19位点检出伴放线菌放线杆菌 ,检出率为 31.6 7% ,在健康部位未检测出伴放线菌放线杆菌 ,二者差异有统计学意义 (P<0 .0 5 ) ;检出伴放线菌放线杆菌的患者的年龄是 (2 8.6 8±7.33)岁 ,未检出伴放线菌放线杆菌的患者的年龄是 (44 .48± 12 .48)岁 ,二者差异有统计学意义 (P <0 .0 1)。结论 :伴放线菌放线杆菌与年轻患者慢性牙周炎的发生、发展密切相关。

?Objective: To determine the distribution of Actinobacillus actinomycetemcomitans (Aa) in subgingival plaques in the patients with chronic periodontitis(CP). Methods: Samples of subgingival plaque were detected for Aa distribution by oligonucleotide probe from 60 sites of CP patients and 10 healthy sites of healthy people.Each sample was obtained from each site of each people. Results: Aa was detected in 19 out of 60 diseased sites(31.67%); Aa wasn't detected in healthy sites.The age(28.68±7.33) of Aa positive patients was younger than that (44.48±12.48) of Aa negative patients( P <0.05).Conclusion: Aa is possibly to cause chronic periodontal infection in younger people.