人膀胱癌基因表达变化的基因芯片研究

Gene expression profile changes in human bladder transitional cell carcinoma

目的 研究膀胱移行上皮细胞癌 (TCC)和正常组织差异表达基因。 方法 应用基因芯片技术对 5例TCC癌组织和正常组织的mRNA进行检测。 结果 在 12 80 0条目的基因中共发现差异表达基因 384条。在癌组织中 87条表达增加 ,2 97条表达降低 ;2 5 9条能在GeneBank中登录。 结论 TCC的发生、发展涉及多基因改变 ,基因芯片技术可同时定量研究大量基因表达水平 ,是一种稳定。

Objective To study the difference in gene expression between human bladder transitional cell carcinoma (TCC) and normal tissue. Methods cDNA microarray was used to detect the mRNA from both 5 TCC patients and the normal tissues. Results Among 12 800 genes there were 384 differences,of which 87 expressed higher and 297 lower in tumor tissue than the normal tissue. Conclusions cDNA microarray technology is a reliable and effective tool in the study of tumor related genes.