超抗原SEA和SEB对正常人TCR V_β基因的限制性取用

Restricted Usage of Human TCR V_β Gene to Superantigen SEA and SEB

金黄色葡萄球菌肠毒素 (SE )作为一种超抗原 ,以MHC非限制性及TCRVβ特异性的方式激活T细胞。本文用定量PCR方法 ,分析SEA和SEB刺激的正常人外周血淋巴细胞T细胞抗原受体Vβ的取用格局。揭示在不同HLA遗传背景下 ,SEA刺激的淋巴细胞均选择性地取用Vβ3和Vβ6两种基因片段 ,而SEB刺激的淋巴细胞则优势表达Vβ2、Vβ8和Vβ9。通过比较分析 ,Sigma产的SEB刺激淋巴细胞后则优势表达Vβ3、Vβ4、Vβ16和Vβ2 0 ,提示外周血淋巴细胞在超抗原作用下发生寡克隆扩增。

As a kind of superantigen, staphylococcal enterotoxins(SE) can activate T cells via non MHC restricted and Vβ specific ways In this study, we analyzed the TCR Vβ usage pattern of normal periphery blood lymphocytes stimulated by three superantigens by quantitative PCR technique As a result, under different HLA genetic background they showed very similar usage patterns But with different sources of same superantigen, the TCR Vβ patterns were quite different Vβ3 and Vβ6 gene fragments were dominantly used by SEA stimulated T cells while oligoclonal proliferation of T cells with Vβ2、Vβ8 and Vβ9 and those with Vβ3, Vβ4, Vβ16, Vβ20 fragments owe to the stimulation of domestic(from Military Science Institute)and foreign SEB(from Sigma), respectively