直肠癌病人外周血NK细胞和T淋巴细胞亚群数目的变化及其临床意义

Alteration and clinical significance of NK cell and T lymphocyte subpopulation in patients with rectal carcinoma

目的 探讨直肠癌病人外周血NK细胞和T淋巴细胞亚群的变化及临床意义。方法 分别应用同位素及流式细胞仪法检测 2 6例正常人 (无肿瘤 )和 35例直肠癌患者 (包括术前术后 )外周血NK细胞和T淋巴细胞亚群数目。结果 NK细胞 ,CD3,CD4,CD4 CD8比值在直肠癌组与对照组差异显著性 (P <0 .0 5 )。直肠癌患者随Dudes分期增高而NK细胞 ,CD3,CD4,CD4 CD8比值逐渐下降 ,差异均有极显著性 (P <0 .0 1)。根治术和姑息性切除术后NK细胞CD3,CD4,CD4 CD8升高 ,CD8回落接近对照组。肿瘤未切除组术后NK细胞 ,CD3,CD4,CD4 CD8则进一步下降 ,差异有显著性 (P <0 .0 5 )。结论 直肠癌患者NK细胞等各项免疫指标大大降低 ,这种免疫紊乱状态在不同病情又有显著差别 。

Objective To investigate the alteration and clinical significance of NK cell and T lymphocyte subpopulatien in patients with rectal carcinoma. Methods The numbers of NK cell and T lymphocyte in 26 healthy persons and 35 rectal carcinoma patients were detected by 3H releasing method and FCM. Results NK cell and CD3, CD4, CD4/CD8 were decreased significantly in patients with rectal carcinoma (P<0.05), which was positively correlated with Dukes staging (P<0.01), and was inereased after redical or palliative operation (P<0.05), but NK cell and CD3, CD4, CD4/CD8 were further decreased in patients with unresectable tumor after operation (P<0.05). Conclusions The immunity of rectal patients was decreased with the development of the disease, and it will be improved after radical of palliative operation.