高温与低剂量香烟联合作用母鼠对仔鼠神经行为发育的影响

Study of nurobehavioral teratogenesis of offspring of pregnant rats exposed to hyperthermia and cigarette smoking

目的 探讨高温与香烟联合对仔鼠神经行为发育的影响。方法 孕d10的大鼠暴露于高温 (4 1℃和 42℃ ) ,同时在孕d2起 ,隔日皮下注射低剂量的香烟烟雾水溶物 (1.0支 /只 ) ,观察分析仔鼠体格发育、反射行为及学习记忆等神经行为。结果 高温组 (4 1℃和 42℃ )仔鼠体格发育 (开眼、张耳、生毛、出牙 )和反射感觉功能 (平面翻正、空中翻正、视觉定向和听觉惊愕 )的达标天数及学习与记忆能力达标时间 (min)与对照组及 37℃组比较明显延迟 (p <0 .0 1) ;同时还表明持续长时间 (>1.5min)的高温与香烟联合对仔鼠上述神经行为具有复合作用 ,与单独高温组比较有明显差异 (p<0 .0 1)。

Objective To study the affection of the hyperthmia and cigarette smoking on the neonate rat's neurobehavioral.Methods Pregnant rats were exposed to high temperature(41℃ or 42℃) on 10*!th day of gestation, meantime water-soluble substances contained cigarette smoking (1.0 cigarette/20g rat) was given through subcutaneous injection once every other day. The neonate rat's neurobehavioral ( the appearance of four physiological markers, the acquisition of four reflexes, learning and memory) was observed. Results In contrast with control group and 37℃ group, when pregnant rats were exposed to high temperature (41℃ and 42℃) the neonate rats were delayed appearantly in four physiologic markers ( eye opening, pinna detachment,hair growth and tooth growth),and days of acquisition of four reflexes(plened righting,air righting,virual placing and auditory orienting)and the time of learning and memory(p<0.01).There was significant combined effect between the sustained hyperthermia in longer time and cigarette smoking , and there was a significant difference compared with the neurobehavioral of those only in single hyperthermia(p<0.01).Conclusion The sustained hyperthermia combined with cigarette smoking can delay,the neurobehavioral development growth of neonate rats.