γ-干扰素诱导角质形成细胞凋亡及其Fas抗原表达研究

Study on IFN-γ Induce Apoptosis of Keratinocytes and Express of Fas Antigen on the Keratinocytes

目的 探讨γ 干扰素在银屑病发病中的作用。方法 通过流式细胞仪测定角质形成细胞中亚二倍体细胞含量、片段化DNA分析及AnnexinV法检测经γ 干扰素诱导的角质形成细胞凋亡 ;采用组织化学、流式细胞仪测定经γ 干扰素作用后的角质形成细胞Fas抗原表达。结果 γ 干扰素上调角质形成细胞Fas抗原表达 (P <0 .0 1) ,诱导角质形成细胞凋亡 (P <0 .0 1)。结论 γ 干扰素可能通过上调角质形成细胞Fas抗原表达 ,进而诱导角质形成细胞凋亡而参与银屑病发病。

Objective To investigate the role of IFN-γ in the pathogenesis of psoriasis.Methods Flow cytometric analysis quantification of hypodiploid cell,analysis of DNA fragmentation,Annexin V method,which study apoptosis of keratinocyte induced by IFN-γ.immunohischemistry and flow cytometry analysis the express of Fas antigen of keratinocyte induced by IFN-γ.Results IFN-γ upregulated the express of Fas antigen on the keratinocytes(P<0.01) and induce apoptosis of keratinocytes(P<0.01).Conclusion IFN-γ upregulated the express of Fas antigen on the keratinocytes and induce apoptosis of keratinocytes,which might be involved in the pathogenesis of psoriasis.