实时荧光定量聚合酶链反应检测解脲支原体感染

Fluorescence Quantitative PCR Detected Infection of Ureaplasma Urealyticum

目的 采用实时荧光定量聚合酶链反应 (FQ PCR)技术 ,准确定量检测待检标本中解脲支原体 (UU )的感染状况。方法 采用实时FQ PCR技术 ,检测标本 765份 ,并用常规PCR技术 ,对比检测了 85例。结果 FQ PCR检测的 765份标本 ,UU DNA阳性 2 79份 ,阳性率 3 6.5 % ,其DNA平均拷贝数为 2 .4× 10 4 。 3 8例阳性患者治疗 2周内复查 ,转阴率为 44.7% ,治疗 3周后复查 ,转阴率 76.0 % (P <0 .0 0 5 )。常规PCR结果重复检测时 ,2 5例阳性者中 2例变为阴性 ,而 60例阴性者中 4例变为阳性。FQ PCR结果重复时完全吻合。结论 实时FQ PCR检测UU是一个较好的方法。

Objective Infection of ureaplasma urealyticum(UU) was detected by real time FQ-PCR technology.Methods 765 cases specimens of UU-DNA quantification were examined by automated of real time quantification with the ABI Prism 7700 Sequece Detector.Comparison detected 85 cases by FQ-PCR and ordinary PCR.Results UU-DNA was found in 279 of 765(36.5%).DNA average amount of positive samples was 2.4×104.38 cases were examined in treatment two week,negative cases 17 of 38(44.7%),after treatment three week,negative cases 29 of 38(76%),P<0.005.2 cases changed from positivity to negativity in 25 cases and 4 cases changed from negativity to positivity in 60 cases when results of ordinary PCR was repeated.Conclusion Real time FQ-PCR is a good method for measuring UU load.