山莨菪碱联用地塞米松对四氧化二氮爆炸致冲毒复合伤大鼠血气的影响

The effect of the anisodaminum and dexamethasone on the changes of blood gas of the rats following the blast-toxicosis combined injury by the explosion of the nitrogen tetroxide

目的 通过复制 N2 O4爆炸致冲毒复合伤模型 ,研究山莨菪碱联用地塞米松对实验动物血气的影响。 方法 采用健康雄性 Wistar大鼠 2 2 4只 ,随机分组 :1模型组 :冲击伤加 N2 O4染毒复合致伤 ;2山莨菪碱组 :伤后即刻、伤后 15 min、伤后 2 h分别腹腔注射山莨菪碱 3mg/ kg,共 3次 ;3地塞米松组 :伤后即刻、15 m in、2 h分别腹腔注射地塞米松 10 mg/ kg,共 3次。4山莨菪碱联用地塞米松组 :伤后分 3次注射山莨菪碱 3mg/ kg及地塞米松 10 mg/ kg。 结果 模型组血氧分压明显下降 ,二氧化碳分压上升 ,血红蛋白氧合能力下降 ,组织氧供下降 ,血液酸化明显。早期腹腔注射山莨菪碱或地塞米松对四氧化二氮冲毒复合伤动物血气变化有一定的抑制作用。而两者联合应用对血气变化有显著的逆转作用。

Objectve To investigate the effects of anisodaminum and dexamethasone on the changes of blood gas of the N 2O 4 blast-toxicosis combined injury model. Methods Two hundred and twenty-four male Wistar rats were divided randomly into 4 groups: ①N 2O 4 blast-toxicosis combined injury model group; ②Anisodaminum treated group; anisodaminum (3 mg/kg) was given ip right after, 15 min after and 2 h after the injury; ③Dexamethasone treated group; dexamethasone (10 mg/kg) was given ip right after the injury, 15 min after and 2 h after the injury; ④Anisodaminum and dexamethasone combine-treated group; anisodaminum (3 mg/kg) and dexamethasone (10 mg/kg) were given ip right after, 15 min after and 2 h after the injury. Each group was subdivided into control group and 6 treatment groups which were killed 3, 6, 12, 24, 48, 72 hours after the injury for blood gas analysis. Results In N 2O 4 blast-toxicosis model group PaO 2 and pH of the plasma were significantly decreased, while PaCO 2 was increased . Combined use of anisodaminum and dexamethasone in the early stage significantly ameliorated the disorders of the blood gas of the combined injury. Conclusion It is suggested that the combined use of anisodaminum and dexamethasone in the early stage is effective in the treatment of blast-toxicosis combined injury caused by the explosion of the nitrogen tetroxide.