肌苷发酵过程的动力学研究

THE KINETIC MODEL FOR THE FERMENTATION PROCESS OF INOSINE

采用枯草杆菌(Bacillus Subtilis)突变株,以葡萄糖为碳源进行间歇发酵研究,得到肌苷发酵过程受底物抑制的动力学关系。在肌苷代谢机理简化的基础上推导建立了工程上适用的菌体增殖动力学方程(dx)/(dt)=0.00266(Sx)/(20.3+s+s^2/76.4+I/6.03)肌苷生成的动力学方程为(dp)/(dt)=0.1791((Sx)/(67.76+s))-8.40(dx)/(dt)基质糖消耗的动力学方程为-(ds)/(dt)=(1/1.017)((dx)/(dt))+(1/0.1207)((dp)/(dt))+(0.3188x)

The Bacillis Subtilis using glucose as carbon source was cultured at 32℃,then inoculated in a six liter stirring fermentor operating at 35℃,400 r/min and vvm=1. The kinetic model for the fermentation process of inosing was obtained.According to the mechanism of the inhibition effect of substrate to bacterium,the rate of multiplication of bacterium is dx/dt=0.00266(s/(20.3+s+s^2/76.4+Ⅰ/6.03)) The rate equation of the consumption of glucose is -(ds/dt)=1/(1.017)dp/dt+1/(0.1207)dp/dt+0.3188x The rate equation of the formation of inosine is ■The specific product formation of inosine and the specific bacterium growth showed that there is a indirect relation between them.So the classification of this fermentation process belongs to the Gaden Ⅱ type,.