检测梅毒的几种试验方法的对比评价

Contrastable Evaluation of Seveal Laboratory Methods for Detecting Treponema Pallidum

目的 探求目前梅毒血清学试验的最佳选择。方法 用 TRUST、TPHA、TP- ELISA、TP- PCR四种方法 ,对 55例血清标本进行对比检测。结果 TRUST法的敏感性为 71 .4% ,特异性为 95.0 % ;TPHA法敏感性和特异性均达 1 0 0 % ;TP- EL ISA及 TP- PCR的敏感性及特异性均低 ,属试剂盒质量问题。结论 建议对初检患者 ,尤其是高危人群 ,宜选用 TPHA法检测 ,以提高初检结果的检出率和可靠性 ,对其结果阳性者 ,再即查 TRUST或 RPR法 ,以随访对比观察疗效。

Aim To search the best serological detection for Treponema Pallidum.Methods Performing contrastable detection on 55 serum samples with the TRUST,TPHA,TP ELISA and TP PCR four methods.Results The sensitivity of TRUST was 71.4% and the specificity was 95.0%.The sensitivity and the specificity of TPHA were both 100%,but the sensitivity and specificity of TP ELISA and TP PCR were both not high for the poor quality of the reagents.Conclusion TPHA is the best choice for patients of first visit to a doctor,especially for the highly dangerous crowd for increasing it is isolated rate.If it is a possitive result,test it again with TRUST or RPR in order to observe the treated effects、relapse and re infection.