血管紧张素Ⅱ对自发性高血压大鼠血红素氧合酶基因表达的影响

The Effect of AngiotensionⅡ on the Heme Oxygenase Gene Expression in Spontaneous Hypertensive Rats

目的 探讨血管紧张素Ⅱ对自发性高血压大鼠 (SHR)动脉壁血红素氧合酶 1(HO 1)基因表达的影响。方法 选取SHR及WKY各 16只 ,分为药物组和对照组 (各 8只 ) ,胃内分别注入培哚普利 [2mg/ (Kg .d) ]或等量生理盐水 14天 ,应用RT PCR技术及放射免疫法分别检测主动脉平滑肌细胞HO 1基因表达和血浆血管紧张素Ⅱ (Ang Ⅱ )的水平。结果 WKY两组的HO 1基因表达与Ang Ⅱ无相关性 ,而SHR两组的HO 1表达与Ang Ⅱ呈负相关。 结论 自发性高血压大鼠体内Ang Ⅱ增高可能抑制HO 1基因的表达。

Objective\ To observe whether the angiotensionⅡ(Ang\|Ⅱ) can affect the heme oxygenase\|1(HO 1) gene expression of aorta smooth muscle cell(SMC) in spontaneous hypertensive rats(SHR). Methods\ 16 SHRs and WKYs were selected and divided into treatment group and control group, respectively. The treated group were feed with perindopril 2mg/kg/d through stomach intubation for 14 days, while control group were given the equivalent volume of normal saline. The HO\|ⅠmRNA expression in aorta smooth muscle cell and the concentration of Ang\|Ⅱ in plasma were measured by RT\|PCR and radioimmunoassay,respectively. Results\ In aorta SMC the expression of HO\|1 mRNA was negatively correlated with the plasma concentrations of Ang\|Ⅱ in treated group and control SHR groups,while no reationship between the two variables was found in WKY rats. Conclusion\ The increase of Ang\|Ⅱ inhibited the expression of HO\|ⅠmRNA of aorta smooth muscle cell in SHR. Perindopril may alleviate the inhibiting effect of Ang\|Ⅱ on HO\|l mRNA.