骨康防治实验性骨质疏松症的骨组织计量学观察

A HISTOMORPHOMETRIC STUDY OF GUKANG ON EXPERIMENTAL POSTMENOPAUSAL OSTEOPEROSIS

为探讨中药对实验性骨质疏松症的防治作用 ,以卵巢切除术制作去势大鼠骨质疏松症的模型 ,采用骨组织形态计量学指标来观察该模型的骨丢失、骨形成与骨吸收的情况 ,评价中药骨康对实验性骨质疏松的防治作用。结果示模型组术后 8周与对照组比较有异常骨丢失 (P<0 .0 1) ,中药组骨丢失程度较模型组明显减轻 (P<0 .0 5 ) ;骨小梁的面积、周长、面积百分比、数量、分离度比较 ,中药组与模型组差异有显著意义 (P<0 .0 5 )。证实本实验动物的造模是成功的 ,中药骨康对防治骨质疏松有显著作用。

Objective: To evaluate the effect of Gukang upon experimental postmenopausal osteoporosis (PMOP). Method: Fourty five female rats were divided into model group, Gukang group, control group at random, except for the latter group, the former two groups were ovariectomized to made models, and the rats in Gukang group were treated by Gukang decoction, while the rats in the other two groups were clystered by cold boiled water. The loss of bone, osteogenesis, and bone absorption were studied by measuring the morphometric parameters of bone tissue. The results showed after 8 weeks of treatment, the loss of bone in model group was more serious than that in control group (P<0.01), while compared with model group, the loss of bone in Gukang group alleviated (P<0.05); there were significant differences in the area, circumference, area percentage, number and separation of bone trabecula between Gukang group and model group (P<0.05). Therefore the ovariectomized models for osteoporosis in this experiment are successful and Gukang has obvious preventive and curative effects on PMOP.