AngⅡ介导的血管平滑肌细胞增殖与迁移效应的实验研究

An Experimental Study on Effect of Proliferation and Migration by Mediated Angiotensin Ⅱ and Vascular Smooth Muscle Cells

目的 :探讨单核细胞趋化蛋白 1单克隆抗体 (MCP 1mcAB)或Losartan拮抗血管紧张素Ⅱ (AngⅡ )介导的血管平滑肌细胞 (VSMCs)增殖与迁移效应的可能性 ,为防治动脉硬化 (AS)提供一定的理论依据。方法 :采用改良的Boyden小室法检测VSMCs的迁移效应 ;以MTT法、3H TdR掺入法、3H 脯氨酸标记法和VSMCs计数评价VSMCs的增殖效应 ;将培养的VSMCs分为 5组 :2 %胎牛血清 ( 2 %FCS)组、AngⅡ组 (其浓度为 10 - 1 0 ～ 10 - 6 Mol/L)、Losartan组 (其浓度为 10 - 7～ 10 - 5Mol/L)、MCP 1mcAb组 (终浓度为 10 μg/ml)和阳性对照组 (含 5 %的酵母多糖活化血清 )。结果 :( 1)AngⅡ具有剂量依赖性地促进VSMCs的增殖与迁移效应 ;( 2 )MCP 1mcAb、Losartan能有效地拮抗AngⅡ介导的VSMCs增殖与迁移效应。结论 :MCP 1mcAb、Losartan拮抗VSMCs的增殖与迁移效应为其防治AS提供一定的理论依据。

Objective: To investigate inverse effect of monocyte chemotactic protein 1 monoclonal antibody(MCP 1 cmAb)or losartan on angiotensin Ⅱ(AngⅡ) mediated proliferation and migration of vascular smooth muscle cell(VSMCs)provids a theoretical base for treatment of atherosclerosis.Method: Effects of proliferation and migration on cultured VSMCs were examed by a modified boyden′s chamber,thiazolyl blue intaking, 3H TdR and 3H proline incorporation as well as VSMCs count in 2% fetal calf serum group(2% FCS group),AngⅡ group,MCP 1 mcAb group and Losartan group.Results: (1)Effects of proliferation and migration on cultured VSMCs were inhanced by dose dependent AngⅡ;(2)Effects of proliferation and migration AngⅡ mediated on VSMCs were inverted by MCP 1 mcAb or losartan.Conclusion: It is suggested that MCP 1 mcAb or losartan play a key role in curing atherosclerotic diseases.