类番茄茄茎段原生质体再生成株的研究

Plant Regeneration from Stem-Derived Protoplasts of Solanum lycopersicoides

利用类番茄茄 (SolamumlycopersicoidesDun .)无菌苗幼嫩茎段酶解获得大量原生质体 (个 ) (2× 10 6/g) ,将原生质体密度稀释至 1× 10 5/mL于HMA培养基中培养 ,则 10d左右出现小细胞团 ,38～ 40d形成肉眼可见的小愈伤组织 (1～ 1.5mm) ,转移至MC增殖培养基 2周后 ,再转移到 15 #分化培养基诱导出芽 ,切取芽体转入5 0P培养基诱发根原基 ,再转入 5 0 #发根培养基形成发达根系 ,成为再生植株 ,整个再生周期 80～ 90d ,再生植株移植至土壤中均能正常生长。

A procedure for protoplast isolation, culture and plant regeneration has been developed for solanum lycopersicoides. Stem-protoplasts were diluted to 1×10 5/mL and cultured in HMA medium, cell colonies (20～30 cells) appeared after 10 days, micro calli (1～1.5mm) formed after 38～40 days from initially culturing. The micro calli were transferred to MC greenig medium for 2 weeks. The green calli were transferred to 15# medium for shoot inducting. The shoots which excised from the callus were transferred to test tubes with 50p medium first for root promoting about 3～4 days and then transferred to 50# rooting medium. Plants regenerated after 80～90 days after initially culturing. All the plants which potted in soil could vigorously grow and blossom.