用RAPD标记分析中国和捷克甘蓝型油菜的遗传多样性

Genetic diversity of Brassica napus L. accessions from China and Czech based on RAPD markers

中国和捷克等 5 0个甘蓝型油菜品种 (系 )的RAPD分析结果表明 ,10个随机引物共扩增出 79条多态带 ,Hamman遗传距离及聚类分析结果与材料的地理起源和系谱关系一致。根据遗传差异将参试材料分为三大组 ,即中国材料组、捷克材料组和中捷材料组。中国材料与捷克材料之间遗传差异最大 ,以中国和捷克交换甘蓝型材料为育种亲本 。

The genetic diveristy and the relationships among a collection of Brassica napus L. accessions, including 20 Chinese, 25 Czech, 2 Germany, 2 France and 1 England, were evaluated using random amplified polymorphic DNA (RAPD) markers. A total of 79 polymorphic amplified products were obtained from 10 primers. The Hamman metric based distance was chosen for which the best fit between a priori and a posteriori grouping was found using UPGMA. Cluster analysis based on Hamman genetic distance placed accessions in the experiment into three main groups. The first group included 16 rape accessions from China and the second group that from Czech with the exception of 'Zhongshuang No.2', a Chinese winter rape. The third group included accessions both from China and Czech. There were genetic variation among experiment accessions, however, the genetic variation between Chinese and Czech accessions was greater than that among Chinese accessions and that among Czech accessions. In view of the obvious genetic differences, the Czech B.napus L. accessions would be an important resources for enriching the genetic background to Chinese rapeseed, and vice versa.