不结球白菜品种(株系)对炭疽病抗性的鉴定与筛选

Identification of non-heading Chinese cabbage cultivars and lines resistant to disease caused by Colletotrichum sp.

报道不结球白菜对炭疽病 (Colletotrichumhigginsianum) 抗性的苗期鉴定方法和抗病品种 (株系 )的筛选结果。在 2片真叶期 ,以 1× 10 6个·mL-1孢子浓度喷雾接种 ,2 5℃、RH >10 0 %保湿 2 4h ,2 5℃光照培育 7d ,可快速地将不同抗性的品种 (株系 )鉴别出来。用该方法所获得的品种 (株系 )抗性鉴定结果与田间苗期 (5片真叶期 )和成株期抗性鉴定结果基本一致。将该方法与田间抗性鉴定相结合 ,从 2 6个品种 (株系 )中筛选出 4个高抗材料 。

The method for identifying resistance of non heading Chinese cabbage to Colletotrichum higginsianum by artificial inoculation of the plant seedlings and screening of the resistant plant varieties and lines by the fast and reliable method were reported.The critical technique of the method included inoculation of seedlings at two leaf stage with a spore suspension of 1×10 6 spores·mL -1 ,and incubation of the inoculated plants in the controlled condition(25 ℃,100% relative humidity)for 24 h and in the greenhouse(25 ℃)for 7 days,respectively,followed by examination of resistance levels.This method allowed for a quick identification of resistance levels of different varieties and lines.Resistance identified by the method was consistent with that identified by the traditional method that uses bigger seedlings(at five leaf stage)and mature plants in the fields.Using this fast and reliable method,four varieties of 26 varieties and lines tested were scored as high resistance.It is suggested that these resistant varieties be used in the agricultural production and breeding.