糖酵解和脂肪酸氧化抑制剂对摘除卵巢猪血清促黄体素和葡萄糖的影响

Influence of inhibitors of glycolysis and fatty acid oxidation on serum concentrations of LH and glucose in ovariectomized pig

研究了急性能量阻断对血清促黄体素和葡萄糖的影响。选用美国佐治亚州雅森斯当地杂交青年母猪 2 0头 ,体重(5 9 7± 10 2 )kg ,12 0～ 130日龄。实验前两周摘除卵巢 ,随机分为 4组 ,每组 5头 :①生理盐水组 (对照 ) ;②糖酵解抑制剂组 (2 脱氧 D 葡萄糖 ,2DG ,40 0mg·kg-1) ;③脂肪酸氧化抑制剂组 (巯基乙酸盐 ,MA ,2 0 0mg·kg-1) ;④ 2DG +MA组。通过颈静脉给药。给药前 2h至给药后 6h经颈静脉导管每 15min采血样一次。次日分离血清后 ,用葡萄糖氧化酶试剂盒定量分析血清葡萄糖浓度 ,用放射免疫分析法 (RIA)定量分析血清促黄体素 (LH)。结果如下 :(1)与对照组比较 ,注射 2DG和 2DG +MA后 ,血清葡萄糖浓度显著升高(P <0 0 5 ) ,而注射MA后未见明显变化。 2DG +MA处理对血清葡萄糖浓度的影响大于 2DG。 (2 )与处理前期相比 ,2DG和 2DG +MA抑制了LH的分泌 ,而生理盐水和MA处理未影响LH的分泌。注射 2DG和 2DG +MA后 ,平均和基础血清LH的浓度低于注射生理盐水组 (P <0 0 2 ) ;但LH的脉冲频率和幅度不受上述处理的影响。实验结果表明 。

Effects of acute loss of energy availability on serum LH and glucose concentrations were studied.Twenty crossbred gilts, (59 7± 10 2)kg BW and 120 to 130 days of age,were ovariectomized and randomly assigned to:①saline vehicle(SAL;n=5),400 mg·kg -1 of the glycolysis inhibitor;②2 deoxy D glucose(2DG,n=5),200 mg·kg -1 of the fatty acid oxidation inhibitor;③mercaptoacetate(MA,n=5);and ④2DG+MA(n=5).Blood was sampled every 15 min for 2 h before and 6 h after treatment iv.Generally,compared to SAL,serum glucose concentrations were greater(P<0 05)after 2DG and 2DG+MA,but not after MA.2DG+MA stimulated a greater(P<0 01)serum glucose response than 2DG alone.Relative to the pretreatment period,2DG and 2DG+ MA suppressed LH secretion,but SAL and MA failed to alter LH secretion.Mean and basal serum LH concentrations were lower(P<0 02)after 2DG and 2DG+MA than after SAL.However,LH pulse frequency and LH pulse amplitude were unaffected by treatments.Thus energy availability could influence pituitary hormone secretion through a sensor within the brain.