摘要
利用随机扩增多态性DNA(RAPD)技术分析了鹅观草属 ( 7种 )、披碱草属 ( 3种 )、猬草属 ( 2种 )和仲彬草属 ( 3种 ) 4个属的 15种植物。对 34个OPRON公司十聚体随机引物进行多态性筛选 ,2 7个 ( 79 4% )能产生多态性。 16个引物产生的 2 13个DNA片断 ,用于计算种间Nei氏遗传相似性系数分析 ,在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明 :①这 4个属的物种各自聚类在一起 ,在相似系数 0 6水平上 ,明显分为 4组 ,分别代表了Hystrix、Elymus、Roegneria和Kengyilia 4个属 ;②Hystrix的 2个物种与Roegneria和Kengyilia物种的亲缘关系较远 ,与同具StH基因组的Elymus也有较大的RAPD变异 ;③Roegneria与Elymus亲缘关系较近 ;④Roegneria中StY和StYH基因组组合的物种亲缘关系很近 ,StY基因组的物种间存在着RAPD遗传变异 ;⑤Kengyilia与Roegneria亲缘关系较近 ;⑥RAPD结果与形态学和细胞学等分析结果一致 ,支持将它们独立作为属分类等级来处理。
Fifteen species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) were analyzed by using random amplified polymorphic DNA (RAPD) markers. 34 decamer oligonucleotides random primers from Opron Technologies were used for polymorphic selection. 27 (79 4%) produced polymorphic products. A total of 213 bands amplified from 16 primers were selected for RAPD analysis. The data were used to generate Nei's similarity coefficients and to construct a dendrogram by using UPGMA in NTSYS programs. It is concluded as follows: ①The species of Roegneria, Elymus, Hystrix and Kengyilia are clustered in four groups, which represent Roegneria, Elymus, Hystrix and Kengyilia respectively on 0 6 of genetic similarity coefficients; ②The relationships between the species of Hystrix and the species of Roegneria and Kengyilia are remote; ③Genetic diversity exists between Elymus and Hystrx although they possess similar StH genome; ④The species of StY and StYH genomes in Roegneria are clustered together, which suggests they have closer relationships; ⑤There is certain RAPD genetic diversity in the species of StY genomes of Roegneria; ⑥Kengyilia is related to Roegneria; ⑦RAPD results are basically comparable with those obtained from studies on morphology and cytology. The present study discusss the usefulness of RAPD markers in the systematic studies of Triticeae.
出处
《四川农业大学学报》
CSCD
2001年第1期14-20,共7页
Journal of Sichuan Agricultural University
基金
四川省教育厅自然科学基金项目
四川省科技厅科研项目
