OPS玻璃化冷冻对山羊卵母细胞超微结构的影响

Uitrastructural changes in goat oocytes cryopreserved by OPS vitrification

对山羊不同发育阶段卵母细胞ＯＰＳ玻璃化冷冻的超微结构损伤进行了电镜观察。结果表明，卵丘细胞除内质网扩张外，未见其他明显的结构损伤；卵母细胞的质膜损伤以ＧＶ期最为严重，其次为培养９ ｈ的，ＩＶＭ 的最轻；ＧＶ期卵几乎看不到微绒毛，而培养９ ｈ和ＩＶＭ卵微绒毛保存较好；ＧＶ期卵母细胞线粒体有的发生了损 伤，主要表现在嵴不清或扩张，培养９ ｈ和ＩＶＭ卵母细胞的线粒体基本上保持了正常的结构；培养９ ｈ卵母细胞的 内质同发生了扩张，ＩＶＭ卵母细胞内质网结构正常。

Ultrastructural damage of goat oocytes at different maturation stage was investigated after OPS vitrification procedure using transmisson electron microscopy (TEM). Cumulus cells keep normal structure except the swelling of endoplasmic reticulum (ER). The oolemma was most seriously damaged in GV oocytes,the second in 9 h,the third in IVM. Microvilli in GV oocytes was often absent,but in 9 h and IVM oocytes, microvilli was well-preserved. Swelling of mitochondrial cristae was seen in GV oocytes.Mitochondriar in 9 h and IVM oocytes was basically well-preserved. Swelling of ER was seen in 9 h oocytes,ER was well-preserved in IVM oocytes.