摘要
本试验利用鸡包涵体肝炎自然病例的肝组织，通过SPF鸡胚连续传代和鸡胚肾细胞培养，分离到一株鸡包涵体肝炎病毒HA株．该病毒株的核酸型为DNA、无束膜、对乙醚、氯仿具有抵抗力；且其对酸有抵抗力，对热敏感。电镜观察发现，病毒粒子呈球形，直径约80～100nm。用2、3、4、5、8型的标准阳性血清迸行中和拭验，证明HA株为血清型2型。回归试验表明HA株具有较强的致病性。
A virus strain (designated Ha) isolated origindry from liver of chicken with inclusion body hepatitis was characterized during chichen embryo kidney cell cultures.The isolated strain contained DNA. The rims was resistant to chlorofrm or ether, could not be inactivated by acids (pH3. 0), was not stalle at 60℃ for 1 hour, It was 80—100um in size. Vivus—neutratazation test in chichen embryo kidney cell culture indicated that Ha was similar to avian admovirus type 2. Typical lesions of inclusion body hepatitis could be produced after the Haisolate was administered orally.
基金
国家自然科学基金
