鼠伤寒沙门氏菌对小鼠IgA、IgM和IL-6产生细胞分布的影啊

Effects of Salmonella typhimurium on the Localisation of IgA,IgM and IL—6 Positive Cells in Mice

用免疫荧光和原位杂交技术检测经口感染鼠伤寒沙门氏菌的C57B16／H2b小鼠脾、小肠肠系膜淋巴结内产生IgA、lgM和IL—6阳性细胞的分布规律。结果表明感染48h后小肠绒毛内lgA阳性细胞数量增多，IgM阳性细胞数量略降低，在小肠粘膜因有层非淋巴滤泡区域强阳性IL—6细胞数量增多，提示IgA产生钿胞在效应区增生和分泌IgA时需某些细胞因子如IL—6的参与，在抗沙门氏菌早期感染中IL—6和IgA有重要作用。

C57B16/H2 mice were administrated orally 103 Salmonella typhimurium and then killed 48 h after challenge. The distribution of IgA,lgM positive B cells and localisation of producing IL—6 cells were investigated using histofluorescence technic and in situ hybridization. The results showed that the number of IgA positive cells in intestinal villi was greatly increased after infection where as IgM positive cell number slightly decreased. Much more number of strongly producing IL—6 cells distributed throughout the non—follicular areas of the intestinal lamina propria(LP). These results demonstrated IgA plasma cell precusors extravasating in LP effector sites are induced to proliferate and secrate IgA depending on some cytokines such as IL—6. IL—6 and IgA antibody were important typhimurium infections.