猪繁殖与呼吸综合征病毒感染抑制猪瘟疫苗的免疫应答

Infection of Porcine Reproductive and Respiratory Syndrome Virus Inhibits Immune Response Against Hog Cholera Virus Vaccine

对 2 0日龄 SPF猪和 2 0日龄猪繁殖与呼吸综合征 ( PRRS)血清阳性猪 ,人工感染猪繁殖与呼吸综合征病毒 ( PRRSV)北京分离株 ( BJ-4 )后 4 8h接种猪瘟疫苗 ,利用 ELISA方法检测仔猪针对 PRRSV和猪瘟疫苗的体液免疫 ,利用 MTS法检测仔猪外周血单核细胞对有丝分裂原 Con A的刺激反应。结果表明 ,不论是SPF仔猪还是带有 PRRSV抗体的仔猪 ,在鼻内接种 PRRSV后 4 8h接种猪瘟疫苗 ,其对猪瘟疫苗的抗体反应显著低于对照组 ,对有丝分裂原 Con A的刺激反应也下降。由此说明 。

To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.Results of serum antibodies detected by ELISA indicated that the infected piglets had specific antibody against PRRSV BJ 4 strain in two weeks,and had significantly lower levels of the antibody against hog cholera vaccine than those of controls.Peripheral blood mononuclear cell(PBMC) from piglets infected with BJ 4 strain showed decreased proliferation responses to mitogen ConA.All results above indicated PRRS virus can suppress both the humoral and cellular immune responses of infected piglets.