肉食兽细小病毒通用PCR诊断技术的建立

Establishment of a Commonly used PCR Technique for Detection of Carnivore Parvoviruses

根据肉食兽细小病毒核苷酸序列高度同源的特点 ,设计合成了 1对引物 ,以犬细小病毒、貂肠炎病毒和猫泛白细胞减少症病毒细胞培养物为 DNA模板 ,进行 PCR特异性片段扩增 ,扩增片段大小为 0 .6kb。结果表明 ,扩增产物与设计的 2个引物之间的序列大小一致。通过通用性、特异性与敏感性试验及对临床送检样品检测 ,证明本法对肉食兽细小病毒通用 ,且具有快速、特异和高度敏感的特点。

Based on the property that the nucleic acid sequence of carnivore parvoviruses was high homologous each other, two primers were designed by means of computer and synthesized. And PCR was established for detection of canine parvovirus, feline panleukopenia virus and mink enteritis virus which was from the isolates of cell culture respectively. The diagnostic technique for these viruses above was proved to be very specific, sensitive and simple.