丹参四倍体优良品系有效成分的含量测定及药理学试验

Determination of effective constituents in autotetraploid elite line of Salvia miltiorrhiza Bunge and primary pharmacological experiments

运用HPLC法测定丹参 (SalviamiltiorrhizaBunge)同源四倍体优良品系 6 1 2 2 2及安徽、江苏和陕西产丹参不同部位、不同生长年限的有效成分 (3种丹参酮和 2种水溶性酚酸 )含量。丹参品系 6 1 2 2 2的有效成分含量明显高于其他产地丹参的含量 ;根中有效成分含量明显高于茎和叶的含量 ;丹参酮和丹参素的含量呈明显相关。初步药理实验结果表明 ,在相同剂量下 ,优良品系 6 1 2 2 2具有更好的药理活性。

Three kinds of tanshinone and two kinds of water\|soluble phenolic\|acid in different parts of plants and different growth years of autotetraploid elite line 61\|2\|22 and different localities of \%Salvia miltiorrhiza \%Bunge were determined by HPLC. The results indicated that the contents of effective constituents in the new autotetraploid of \%Salvia miltiorrhiza \%were significantly higher than that in other samples. The contents of effective constituents in roots were significantly higher than that in stems and leaves. The content of tanshinone was closely related to the content of danshensu in all tested materials. Primary pharmacological experiments indicated that the new autotetraploid line of \%Salvia miltiorrhiza \%showed more active pharmacological effects.