5种石斛及其组织培养物对活性氧的清除作用

The scavenging of reactive oxygen species by crude drugs and cultured tissues of five species of Dendrobium Sw.

采用化学发光法 ,以 3种活性氧ROS(O2÷、·OH、H2 O2 )模型检测 5种药用石斛及其培养物对ROS的清除作用。5种药用石斛及其培养物的氯仿与甲醇提取物均具有清除 3种ROS的作用 ,且多超过阳性对照Vc的清除能力。氯仿提取物中以杯鞘石斛 (DendrobiumgratiosissmumRchb .f.)药材的清除ROS能力最强 ,甲醇提取物中以铁皮石斛(D .officinaleKimura)培养物的清除ROS能力最强。

Using chemiluminescence system for detecting ability of scavenging reactive oxygen species ROS (O\-\{2\}\+\{÷\}、·OH、H\-\{2\}O\-\{2\}) in five species of \%Dendrobium \%Sw. The results show that most of chloroform and methanol extracts from crude drugs and cultured tissues have significant scavenging effect on ROS and many of them are higher than positive control (Vc). The chloroform extract from \%Dendrobium gratiosissmum \%Rchb. f. (crude drug) and methanol extract from \%D. officinale \%Kimura (cultured tissues) have the best scavenging of ROS ability. This results indicated that \%Dendrobium \%Sw. are good natural drugs for scavenging of ROS.