半夏种内不同居群酯酶和超氧化物歧化酶同工酶酶谱特征分析

Analysis on characteristic zymogram phenotypes of easterase and superoxide dismutase from the laminas in populations of Pinellia ternata (Thunb.) Breit.

对栽培在同一生境下的 16个半夏〔Pinelliaternata (Thunb .)Breit.〕居群同一生长期叶片中酯酶 (EST)和超氧化物歧化酶 (SOD)同工酶酶谱进行了比较分析 ,结果表明 :EST和SOD同工酶酶谱在各居群间 ,甚至在同一居群内除少数共有的特征谱带外存在着较为明显的频率差异。EST同工酶在半夏各居群中最多可显示 12条谱带 ,其中在慢区的第 5 (弱带 )和快区的第 10 (强带 ) 2条谱带为各居群所共有的特征谱带 ;SOD同工酶在半夏各居群中最多显示 9条谱带 ,其中在慢区的第 2 (弱带 )、快区的第 5 (强带 )和第 9(强带 )

Sixteen populations of \%Pinellia ternata \%(Thunb.) Breit. originated mainly from middle and lower reaches of Yangtze River valley were collected and cultivated in Weigang Experimental Farm of Nanjing Agricultural University with same cultural method, which zymogram phenotypes of easterase(EST) and superoxide dismutase(SOD) from the laminas sampled in same growth period were compared by using PAGE. The results showed that the populations, even individuals in same population could be distinguished from each other by their zymogram phenotypes of EST and SOD with apparent frequency diversity, except some characteristic bands. Twelve bands of EST were found at most among the different populations, including two characteristic bands appeared in all populations, i.e. the fifth weak band in slow region and the tenth stronger band in fast region. Nine bands of SOD were found at most among the different populations, including three characteristic bands appeared in all populations, i.e. the second weak band in slow region and the fifth and ninth stronger band in fast region.