核黄素电化学还原研究

Studies on Electroreduction Mechanism of Riboflavin

本文采用循环伏安、本体电解、荧光光谱、电子自旋共振波谱等技术对核黄素电化学还原机理进行了研究.结果表明,在 DMSO 溶液中,核黄素以质子化和非质子化两种形式存在,它们的还原电位分别是-0.78和-1.05V(vs·SCE).两种形式的核黄素都可在汞电极上得到一个电子,生成相应的自由基.自由基的 g 值分别为2.005和2.002.对质子化式的自由基的 ESR 超精细谱进行了理论分析,提出了核黄素可能的电化学还原机理.

Electrochemical reduction of riboflavin was studied by means of cyclic voltammetry,bulk electrolysis,luminescence spectra and ESR spectroscopy jointed with electrochemicaltechniqus.The results revealed that there were a protonated and a nonprotonated forms ofriboflavin,which were reduced at -0.78 and -1.05V respectively producing theircoresponding radicals.The respective g values of the radicals are 2.005 and 2.002.Atheoratical analysis of the hyperfine ESR spectra of the protonated radicals of the titledcompound was made.Hence the possible electroreduction mechanism of riboflavin hasbeen proposed.