激光扫描共聚焦显微镜技术的发展及应用

Development of Confocal Microscopy and Its Application

激光扫描共聚焦显微术是先进的分子和细胞生物学研究技术。它在荧光显微镜成像的基础上加装激光扫描装置 ,结合数据化图像处理技术 ,采集组织和细胞内荧光标记图像 ,在亚细胞水平观察钙等离子水平的变化 ,并结合电生理等技术观察细胞生理活动与细胞形态及运动变化的相互关系。由于它的应用范围较广泛 ,已成为形态学、分子细胞生物学、神经科学和药理学等研究领域中很重要的研究技术。

Confocal laser scanning microscopy is an advanced technology for molecular and cellular biology. Based on the fluorescent microscopy, the laser scanning equipment and digital image analysis, confocal microscopy has been applied to collect the fluorescence labeled images of the tissues and cells, analyze the change in the levels of ions at subcellular level, and visualize the correlationes among physiology, morphology and the cellular mobility in combination with electrophysiology. The broad range of the application makes confocal laser scanning microscopy to be a very important technique in the studies of the morphology, molecular and cellular biology, neuroscience and pharmacology.