摘要
利用差示脉冲溶出伏安技术和叶酸在电极表面的吸附特性 ,建立了测定痕量叶酸的电化学分析方法 .方法具有快速、简便、灵敏度高、干扰少的优点 .在不富集的实验条件下 ,差示峰电流与叶酸浓度在 3 39× 1 0 - 8～ 3 73× 1 0 - 7mol/L范围内呈良好线性关系 ;而在 - 0 2 5V电位吸附富集 70s条件下 ,其线性范围为 6 78×- 1 0 ～ 2 2 7× 1 0 - 8mol/L ,检测限达 1 0× 1 0 - 1 1 mol/L。将该方法应用于小米和玉米中微量和痕量叶酸的测定 ,结果满意 ,回收率在 94%～ 1 0 2
Electrochemical analysis is used to determine trace folic acid by Different Pulse Stripping Voltammetry and to absorb properties of folic acid in electrode face.This method has the characteristics of being fast,used easily,high sensitivity and little interference.The peak currents have linear relation with folic acid concentration in the range 3 39×10 -8 ～3 73×10 -7 mol/L without preconcentration,and 6 78×10 -10 ～2 72×10 -8 mol/L with preconcentration for 70S under-0.25V.The detect limit is 1 0×10 -11 mol/L.This method has satisfactory result in determining trace folic acid in millet and maize.Its recovery rate is between 94% and 102%.
基金
河南省科委自然科学基金
杰出青年基金资助课题!(9940 3 0 70 0
99112 0 115 )
关键词
差示脉冲溶出伏安法
叶酸
玉米
小米
粮食
测定
Different Pulse Stripping Voltammetry
folic acid
maize
millet
electrochemical analysis
