超临界CO_2萃取精馏EPA与DHA的实验研究

Experiment on Supercritical Carbon Dioxide Extraction—Fractionation of EPA and DHA

采取超临界 CO2萃取—精馏技术，对经尿素包合预处理的鱼油脂肪酸乙酯在超临界 CO2流体中的溶解情况进行了考察，探索了 EPA、 DHA的分离提纯工艺，并讨论了实验的影响因素。实验结果表明：采取温度梯度结合逐步升压法，能使鱼油脂肪酸乙酯按碳链长度依次分离， EPA＋ DHA提纯至 90％，两次分离后， EPA提纯至 67％， DHA提纯至 90％以上。

Experiment on supercritical carbon dioxide extraction— fractionation of fish oil fatty acid ethyl esters(FAEE) which had been enriched by urea crystallization was carried out. Solubility of fish oil fatty acid ethyl esters in SC－ CO2 was measured ,and technical conditions of purity for EPA and DHA were researched. Moreover, effects on experimental results were concluded. The results showed FAEE could be separated by chain length with the method of combination of increasing pressure gradually and keep a temperature gradient. With two separation, EPA and DHA can be concentrated to 67％ and above 90％ respectively.