钴(Ⅱ)-5-(4-磺基-1-萘偶氮)-8-羟基喹啉-OP 显色体系的分光光度法研究及应用

Study on the spectrophotometric method of Cobalt(Ⅱ)-5-[(4-Sulfo-1-naphthyl) azo]-oxine-surface active agent OP system and its application

本文研究了5-(4-磺基-1-荼偶氮)-8-羟基喹啉在表面活性剂 OP 存在下与钴(Ⅱ)的显色反应的条件。试验结果表明,在 pH5. 6～6. 6的 KH_2PO_4-NaOH 缓冲溶液和 OP 存在下,钴(Ⅱ)与该试剂能形成稳定的红色配合物。其λ_(max)=510nm,ε_(510) =6. 69×10~4L·mol^(-1) ·cm^(-1) ,钴(Ⅱ)∶试剂=1∶3,钴(Ⅱ)含量在0～0. 7μg/ml 范围内符合比尔定律。本文所拟定的分析方法用于维生素 B_(12) 中微量钴的测定,获得满意结果。

In this paper,a new chromogenic reaction of Cobalt(Ⅱ)with 5- [(4-Sulfo-1-naphthyl)azo]-oxine and surface-active agent OP system is investigated.In the surcface-active agent OP and at pH5. 6-6. 6 bu- ffer solution,the reagent reacts with Co(Ⅱ)to form a red complex. λ(?)=510nm,ε_(510) =6. 69×10~4L·mol^(-1) ·cm^(-1) .Co(Ⅱ):Reagent=1:3. Beer's Law is obeged for Co(Ⅱ)in the range of 0-0. 7μg·ml^(-1) .The method has been applied to the determination of Cobalt(Ⅱ)Injection VitaminB_(1 2) .The results are satisfactory.