摘要
目的 :观察小鼠重组Periostin对成骨细胞附着和伸展的作用。方法 :采用固相结合分析实验和四唑盐比色 (MTT)法等细胞生物学技术 ,观察成骨细胞在Periostin基质上的附着能力和伸展率。结果 :成骨细胞在Periostin基质上附着和伸展良好 ,当浓度在 2 0 μg/ml以上时 ,作用尤其显著 (P <0 .0 1) ,与在纤维结合蛋白 (Fn)基质上生长相似。结论 :小鼠重组Periostin可有效促进成骨细胞的附着和伸展 ,但其作用的确切分子机制还需深入研究。
AIM: To investigate the effects of mouse recombinant Periostin on adhesion and spread of osteoblast. METHODS: Solid-phase binding assay and MTT method were used to observe the ability of adhesion and spread of osteoblast on Periostin matrix. RESULTS: Adhesion and spread of osteoblast are very well on Periostin at dose of 20μg/ml or more ( P <0.01) and the effects of Periostin are similar to FN. CONCLUSION: Periostin can promotes the adhesion and spread of osteoblast, but the mechanism needs further study. [
出处
《牙体牙髓牙周病学杂志》
CAS
2001年第3期189-191,共3页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助课题!( 3 970 0 164 )
