新流行期汉城型汉坦病毒全S及部分L与M基因核苷酸序列测定及研究

S SEGMENT AND PARTIAL L.M SEGMENT NECLEOTIDE SEQUENCING OF A NECOLY EPIDEMIC SEOUL TYPE VIRUS.

目的 :为获得 SEO型病毒基因组更为详尽的资料 ,也为局部爆发的原因提供科学的资料。方法 :病毒分离、Mc Abs分型 RT- PCR扩增及核苷酸序列测定。结果 :首次测出我国 SEO型病毒较为完整的全 S片段及部分 L 片段和 M片段并与 76 - 118、Seoul、SR1 1 (在全 S片段 ) ;76 - 118、C4、L99、Seoul(在 L 片段 ) ;10 A、A9、C1 - 1 、JB3、L99、SD2 2 7、SD70 、SEO(M片段 )株核苷酸同源性进行比较 ,分别为 6 4.2 %、 91.89%、 96 .8%及 70 .8%、 72 .0 %、 95 .3%、 95 .9%及 97.3%、6 3.0 %、 6 6 .0 %、 93.0 %、 94.3%、 96 .3%、 97.3%、 94.7推导出的氨基酸同源性分别为 :80 .5 %、 95 .4%、 97.36 %及 75 .6 %、75 .1%、 97.7%、 97.36 %及 96 .0 %、 78.8%、 79.8%、 94.9%、 98.0 %、 97.0 %、 98.0、 97.0 %。结论 :1不存在病毒基因组间的重排 ,即基因重排不是造成此次 HFRS流行的原因。 2 SEO型病毒表现出明显的地理聚集现象。

Objective:Epidemiologic features of hemorrhagic fever with renal syndrome(HFRS) is endemic outbreak of SEO type nowadays.This article aims to study SEO viral genes and the cause of endemic outbreak of HFRS.Methods:Viral isolation、McAbs typing and RT-PCR were used to identify a SEO strain from hebei province in 1996,nucleotide sequences of its S gene was detected.Results:Necleotide sequences of the whole S segment and partial L.M segment of SEO virus were determined in China for the first time.When compared with the whole S segment of 76-118,Seoul,SR11 strains,partial L segment of 76-118,C 4,L 99 ,Seoul strains and partial M segnient of LoA、Aq Ch,JB3,L99,SD227,SD70 strains,the homology of necleotide sequences of the new isolate to other strains was 64 2%,91 8%,96 8% and 70 8%,72 0%,95 3%,95 9% and 97 3%,63 0%,66 0%,93 0%,94 3%,96 3%,97 3%,94 7%,respectively.The homology of amino acid was 80 5%,95 4%,97 3% and 75 6%,75 1%,97 7%,97 36% and 96 0%,78 8%,79 8%,94 9%,98 0%,97 0%,98 0%,97 0% respectively.Conclusion:There was no reassortment of viral genes.Reassortment wasnt the cause of this HFRS epidemic.SEO type virus has apparent endemicity.Selecting the same vaccine to local epidemic virus strain was an effective prerentive measure.