摘要
目的 :为获得 SEO型病毒基因组更为详尽的资料 ,也为局部爆发的原因提供科学的资料。方法 :病毒分离、Mc Abs分型 RT- PCR扩增及核苷酸序列测定。结果 :首次测出我国 SEO型病毒较为完整的全 S片段及部分 L 片段和 M片段并与 76 - 118、Seoul、SR1 1 (在全 S片段 ) ;76 - 118、C4、L99、Seoul(在 L 片段 ) ;10 A、A9、C1 - 1 、JB3、L99、SD2 2 7、SD70 、SEO(M片段 )株核苷酸同源性进行比较 ,分别为 6 4.2 %、 91.89%、 96 .8%及 70 .8%、 72 .0 %、 95 .3%、 95 .9%及 97.3%、6 3.0 %、 6 6 .0 %、 93.0 %、 94.3%、 96 .3%、 97.3%、 94.7推导出的氨基酸同源性分别为 :80 .5 %、 95 .4%、 97.36 %及 75 .6 %、75 .1%、 97.7%、 97.36 %及 96 .0 %、 78.8%、 79.8%、 94.9%、 98.0 %、 97.0 %、 98.0、 97.0 %。结论 :1不存在病毒基因组间的重排 ,即基因重排不是造成此次 HFRS流行的原因。 2 SEO型病毒表现出明显的地理聚集现象。
Objective:Epidemiologic features of hemorrhagic fever with renal syndrome(HFRS) is endemic outbreak of SEO type nowadays.This article aims to study SEO viral genes and the cause of endemic outbreak of HFRS.Methods:Viral isolation、McAbs typing and RT-PCR were used to identify a SEO strain from hebei province in 1996,nucleotide sequences of its S gene was detected.Results:Necleotide sequences of the whole S segment and partial L.M segment of SEO virus were determined in China for the first time.When compared with the whole S segment of 76-118,Seoul,SR11 strains,partial L segment of 76-118,C 4,L 99 ,Seoul strains and partial M segnient of LoA、Aq Ch,JB3,L99,SD227,SD70 strains,the homology of necleotide sequences of the new isolate to other strains was 64 2%,91 8%,96 8% and 70 8%,72 0%,95 3%,95 9% and 97 3%,63 0%,66 0%,93 0%,94 3%,96 3%,97 3%,94 7%,respectively.The homology of amino acid was 80 5%,95 4%,97 3% and 75 6%,75 1%,97 7%,97 36% and 96 0%,78 8%,79 8%,94 9%,98 0%,97 0%,98 0%,97 0% respectively.Conclusion:There was no reassortment of viral genes.Reassortment wasnt the cause of this HFRS epidemic.SEO type virus has apparent endemicity.Selecting the same vaccine to local epidemic virus strain was an effective prerentive measure.
出处
《现代预防医学》
CAS
2001年第2期132-135,共4页
Modern Preventive Medicine
关键词
肾综合征出血热
汉坦病毒
核苷酸序列
Hemorrhagic fever with renal syndrome(HFRS)
Hantaan virus
Nuclotide sequence