急性脑梗死患者红细胞内外Ca^(2+)、ATP含量及膜Ca^(2+)-Mg^(2+)ATPase活性变化的动态研究

Dynamic stuey on intracellular free calcium,ATP level and membrane Ca^(2+)-Mg^(2+) ATPase activity of erythrocyte in patients with acute cerebral infarction

目的 探讨脑梗死 (CI)患者急性期红细胞 (RBC)内外 Ca2 + 、ATP含量及膜 Ca2 + - Mg2 + ATPase活性变化的规律。方法 用 FACS Vantage流式细胞仪检测 30例急性期 CI患者及 2 8名健康对照者 RBC内游离 Ca2 + 浓度 ([Ca2 + ]i)、ATP含量及 RBC膜 Ca2 + - Mg2 + ATPase活性 ,并进行动态观察。结果 CI组 RBC[Ca2 + ]i较对照组显著升高 (P <0 .0 1) ;而 ATP含量、膜 Ca2 + - Mg2 + ATPase活性均较对照组明显降低 (分别为 P<0 .0 5、P<0 .0 0 1)。上述结果老年组较青年组明显。CI组 RBC[Ca2 + ]i:发病第 1～ 2天即明显升高 ,3～7天最高 ,于 8～ 15天逐渐降至较发病 1～ 2天时稍低水平 ,但仍比对照组高。而 RBC内 ATP含量、膜 Ca2 + -Mg2 + ATPase活性在发病第 1～ 2天即明显下降 ,3～ 4天降至最低值 ,1周后两者均略有回升。 CI患者 RBC[Ca2 + ]i分别与膜 Ca2 + - Mg2 + ATPase、ATP含量呈明显负相关 (r=- 0 .90 4、r=- 0 .978,均 P<0 .0 5 ) ;RBC内 ATP含量与膜 Ca2 + - Mg2 + ATPase活性之间呈正相关 (r=0 .835 ,P<0 .0 5 )。结论 CI急性期存在 RBC内钙超载 ,RBC内 ATP含量、膜 Ca2 + - Mg2 + ATPase活性也参与了 RBC内钙超载的病理过程 。

Objective To explore the dynamic alteration of intracellular free calcium concentration([Ca 2+ ]i),ATP level and membrane Ca 2+ Mg 2+ ATPase activity of erythrocyte in the patients with acute cerebral infarction(CI).Methods we examined [Ca 2+ ]i,ATP level and membrane Ca 2+ Mg 2+ ATPase activity of erythrocyte in 30 patients with acute CI and 28 health controls by Fluorescence Activated Cell Sorter.Results [Ca 2+ ]i in erythrocyte increased significantly in CI group( P <0.01),while the ATP level and membrane Ca 2+ Mg 2+ ATPase activity were lower than the controls( P <0.05, P <0.001).The above result was more remarkable in the elderly group than the young one.The dynamic alteration of [Ca 2+ ]i in erythrocyte increased obviously during 1~2 days after the attack,and reached the peak in 3~7 days,it decreased slowly to the slightly low level at the beginning of the attack in about two weeks,but it was still higher than the controls.The dynamic alteration of ATP level and Ca 2+ Mg 2+ ATPase activity after acute CI,it decreased significantly during 1~2 days after the attack,and reached the lowest in 3~4 days.this status could last about one week.Then both of them increased slightly. There was remarkable negative correlation between RBC [Ca 2+ ]i and ATP level or membrance Ca 2+ Mg 2+ ATPase activity ( r =-0.904, r =-0.978, P <0.05).There was positive correlation between ATP level and membrane Ca 2+ Mg 2+ ATPase activity( r =0.835, P <0.05).Conclusion There was calcium overload [Ca 2+ ]i in the intracellular of erythrocyte in acute CI,ATP level and membrane Ca 2+ Mg 2+ ATPase activity of erythrocyte CI was involved in the pathological course of calcium overload,and related to the age.