持续性CO_2气腹影响胃癌细胞在腹腔镜切口种植的实验研究

Effect of continuous CO_2 pneumoperitoneum on tumor cell port site implantation in laparoscopic surgery in a murine model

目的 观察实验大鼠CO2 气腹对肿瘤细胞切口播散的影响。方法 在雄性SD大鼠腹腔内注入不同量人胃癌细胞株SGC 790 1,用气腹机分别产生不同压力的持续性气腹 ,通过 5mm腹腔穿刺管收集气体至培养瓶中 ,在 37℃、5 %CO2 环境中培养 7d后 ,鉴定是否有肿瘤细胞生长 ,另取腹腔冲洗液作阳性对照。结果 注入 10 6/ml浓度的胃癌细胞 ,气腹压达 30mmHg ,流量为 5L/min ,持续 6 0min、12 0min及 180min后 ,部分培养瓶中有肿瘤细胞生长 (P <0 0 1) ,而在注入 10 4 /ml胃癌细胞所收集的气体中未发现肿瘤细胞。结论 在 10 6/ml肿瘤细胞数量下 。

Objective To determine whether the pressure of continuous CO 2 pheumoperitoneum (CCP) affects port site implantation of tumor cells.Methods Male SD rats were injected intraperitoneally with gastric cancer cells (cell line SGC 7901). Continuous CO 2 pneumoperitoneum at 15*!mm*!Hg or 30*!mm*!Hg for 5*!mins,60*!mins,120*!mins and 180*!mins with different number of tumor cell injection(10 4/ml, 10 6/ml respectively) was then established. Several samples of peritoneal washing served as positive control.All collecting dishes were incubated at 37℃ for one week.Results Dishes were tumor cell positive on which the tumor cells were collected from rats undergoing continuous flows of CO 2 gas (5*!L/min) at pneumoperitoneum of 30*!mm*!Hg for 60 mins or longer and all peritoneal washing samples showed tumor growth also. Conclusion It suggests that the number of tumor cells, pneumoperitoneum pressure and the duration may affect the port site implantation.